Metagenomics of highly enriched PAO biomass from lab-scale EBPR SBR s... - SRA

SRX5548959: Metagenomics of highly enriched PAO biomass from lab-scale EBPR SBR system
1 ION_TORRENT (Ion Torrent PGM) run: 1.6M spots, 453.8M bases, 344.4Mb downloads

Design: Ion Torrent PGM sequencing of biomass enriched using density-based centrifugation; libraries prepared using NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent; fragments selected at 400-450bp using 2% Size Select E-Gel and sequenced on Ion Torrent 316 v2 chip and Ion OneTouch 400 Template/Sequencing Kit v2

Submitted by: Columbia University

Study: Metagenome-derived genomes from bioreactors highly enriched in phosphorus- and glycogen-accumulating organisms

PRJNA528323 • SRP188997 • All experiments • All runs

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Biomass from three sequencing batch reactors (SBRs) individually enriched in phosphorus-accumulating organisms (PAO) Candidatus 'Accumulibacter phosphatis' (CAP) clades IC and IIC and glycogen-accumulating organism (GAO) Candidatus 'Competibacter phosphatis' lineage 4 were further enriched using density-based centrifugation. Enrichment was confirmed using DGGE before and after centrifugal separation. Ion Torrent PGM sequencing, non-taxonomic binning was used to assemble high-quality metagenome-derived genomes for CAP IC, CAP IIC, and CCP lineage 4.

Sample: SBR_L

SAMN11179414 • SRS4514539 • All experiments • All runs

Organism: Candidatus Accumulibacter contiguus

Library:

Name: SBR_L PAO6

Instrument: Ion Torrent PGM

Strategy: WGS

Source: METAGENOMIC

Selection: size fractionation

Layout: SINGLE

Runs: 1 run, 1.6M spots, 453.8M bases, 344.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR8758396	1,622,571	453.8M	344.4Mb	2020-04-01

ID:: 7479793

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